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Primer3 is an open-source tool used in molecular biology to design high-quality PCR primers. It optimizes critical thermodynamic characteristics to ensure highly specific DNA amplification. Step 1: Retrieve Your Target Sequence

Before using the tool, you must gather your template sequence:

Source: Search databases like the NCBI Gene Database to find your target organism and locus.

Format: Extract the nucleotide sequence in FASTA format (the raw sequence text preceded by a “>” title line). Step 2: Input Your Data into Primer3 How to design primers for your PCR reaction | Primer3